Journal: Bioengineered

Article Title: MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4

doi: 10.1080/21655979.2021.1871817

Figure Lengend Snippet: RIPK4 was a direct target of miR-330-3p in ES-2 cells. (a) The binding sites between RIPK4 and miR-330-3p predicted by TargetScan. (b)The binding relationship of RIPK4 to miR-330-3p validated by dual-luciferase reporter gene assay, * P < 0.05. The expression level of RIPK4 was detected using RT-qPCR (c) and western blot (d-e), * P < 0.05

Article Snippet: Primary antibodies against RIPK4 (ab203541, Abcam, Cambridge, UK) and GAPDH (ab8245, Abcam, Cambridge, UK) were utilized in this study.

Techniques: Binding Assay, Luciferase, Reporter Gene Assay, Expressing, Quantitative RT-PCR, Western Blot

Journal: Bioengineered

Article Title: MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4

doi: 10.1080/21655979.2021.1871817

Figure Lengend Snippet: Overexpression of RIPK4 antagonized the effects of miR-330-3p on ES-2 cells. The inhibition effect of miR-330-3p on RIPK4 expression was rescued by RIPK4 overexpression detected using RT-qPCR (a) and western blot (b), * P < 0.05. The inhibition effect of miR-330-3p on cell proliferation, migration and invasion was partly reversed by RIPK4 overexpression detected using MTT (c) and transwell assay(d), * P < 0.05

Article Snippet: Primary antibodies against RIPK4 (ab203541, Abcam, Cambridge, UK) and GAPDH (ab8245, Abcam, Cambridge, UK) were utilized in this study.

Techniques: Over Expression, Inhibition, Expressing, Quantitative RT-PCR, Western Blot, Migration, Transwell Assay

Journal: Bioengineered

Article Title: MicroRNA miR-330-3p suppresses the progression of ovarian cancer by targeting RIPK4

doi: 10.1080/21655979.2021.1871817

Figure Lengend Snippet: The expression of RIPK4 was inversely correlated with miR-330-3p expression in OV tissues

Article Snippet: Primary antibodies against RIPK4 (ab203541, Abcam, Cambridge, UK) and GAPDH (ab8245, Abcam, Cambridge, UK) were utilized in this study.

Techniques: Expressing

Journal: BioMed Research International

Article Title: Overexpression of RIPK4 Predicts Poor Prognosis and Promotes Metastasis in Ovarian Cancer

doi: 10.1155/2021/6622439

Figure Lengend Snippet: The differentially expressed genes obtained from TCGA and the GTEx databases. (a) Volcano plot representing obtained genes. Red dots denote upregulated genes, green dots denote downregulated genes, and black dots denote nonsignificant genes. (b) Heat map of the top 50 relevant genes selected after screening. (c) Scatter diagram displaying RIPK4 expression between ovarian cancer (red dots) and normal ovarian tissues (green dots) ( P value < 0.001).

Article Snippet: The membranes were blocked with Tris-buffered saline with 0.1% ( v / v ) Tween-20 (TBST) containing 5% ( w / v ) nonfat milk for an hour at room temperature and incubated with primary antibodies for RIPK4 (A8495; 1 : 1000; ABclonal Technology, Wuhan, China), β -catenin (ab16051; 1 : 1000; Abcam, Cambridge, MA, USA), or β -actin (1 : 1000; Proteintech, Chicago, IL, USA) overnight at 4°C.

Techniques: Expressing

Journal: BioMed Research International

Article Title: Overexpression of RIPK4 Predicts Poor Prognosis and Promotes Metastasis in Ovarian Cancer

doi: 10.1155/2021/6622439

Figure Lengend Snippet: Relationship between RIPK4 expression and clinical-pathological characteristics. (a) Clinical stage. (b) Distant metastasis. (c, d) The wound healing assay in SKOV3 and HO8910 cells after transfection. (e, f) The transwell assay in SKOV3 and HO8910 cells.

Article Snippet: The membranes were blocked with Tris-buffered saline with 0.1% ( v / v ) Tween-20 (TBST) containing 5% ( w / v ) nonfat milk for an hour at room temperature and incubated with primary antibodies for RIPK4 (A8495; 1 : 1000; ABclonal Technology, Wuhan, China), β -catenin (ab16051; 1 : 1000; Abcam, Cambridge, MA, USA), or β -actin (1 : 1000; Proteintech, Chicago, IL, USA) overnight at 4°C.

Techniques: Expressing, Wound Healing Assay, Transfection, Transwell Assay

Journal: BioMed Research International

Article Title: Overexpression of RIPK4 Predicts Poor Prognosis and Promotes Metastasis in Ovarian Cancer

doi: 10.1155/2021/6622439

Figure Lengend Snippet: The relationship between RIPK4 expression a and clinicopathological characteristics.

Article Snippet: The membranes were blocked with Tris-buffered saline with 0.1% ( v / v ) Tween-20 (TBST) containing 5% ( w / v ) nonfat milk for an hour at room temperature and incubated with primary antibodies for RIPK4 (A8495; 1 : 1000; ABclonal Technology, Wuhan, China), β -catenin (ab16051; 1 : 1000; Abcam, Cambridge, MA, USA), or β -actin (1 : 1000; Proteintech, Chicago, IL, USA) overnight at 4°C.

Techniques: Expressing

Journal: BioMed Research International

Article Title: Overexpression of RIPK4 Predicts Poor Prognosis and Promotes Metastasis in Ovarian Cancer

doi: 10.1155/2021/6622439

Figure Lengend Snippet: Verification of the efficiency of the prognostic prediction model in patients with ovarian cancer. (a) RIPK4 expression is associated with overall survival in patients with ovarian tumor. (b) The overall survival rate in the high- and low-risk groups according to the prognostic prediction model. (c) The time-dependent receiver operating characteristic curves at different years. (d) A heat map representing the age, stage, and RIPK4 expression for every patient with ovarian tumor based on The Cancer Genome Atlas data.

Article Snippet: The membranes were blocked with Tris-buffered saline with 0.1% ( v / v ) Tween-20 (TBST) containing 5% ( w / v ) nonfat milk for an hour at room temperature and incubated with primary antibodies for RIPK4 (A8495; 1 : 1000; ABclonal Technology, Wuhan, China), β -catenin (ab16051; 1 : 1000; Abcam, Cambridge, MA, USA), or β -actin (1 : 1000; Proteintech, Chicago, IL, USA) overnight at 4°C.

Techniques: Expressing

Journal: BioMed Research International

Article Title: Overexpression of RIPK4 Predicts Poor Prognosis and Promotes Metastasis in Ovarian Cancer

doi: 10.1155/2021/6622439

Figure Lengend Snippet: Univariate and multivariate analyses representing the correlation between clinicopathological characteristics and overall survival in patients with ovarian cancer.

Article Snippet: The membranes were blocked with Tris-buffered saline with 0.1% ( v / v ) Tween-20 (TBST) containing 5% ( w / v ) nonfat milk for an hour at room temperature and incubated with primary antibodies for RIPK4 (A8495; 1 : 1000; ABclonal Technology, Wuhan, China), β -catenin (ab16051; 1 : 1000; Abcam, Cambridge, MA, USA), or β -actin (1 : 1000; Proteintech, Chicago, IL, USA) overnight at 4°C.

Techniques:

Journal: BioMed Research International

Article Title: Overexpression of RIPK4 Predicts Poor Prognosis and Promotes Metastasis in Ovarian Cancer

doi: 10.1155/2021/6622439

Figure Lengend Snippet: Enrichment results from GSEA. (a) The results of the top 10 relevant gene sets in the GO analysis. (b) The results of the top 10 relevant pathways in the KEGG analysis. (c, d) The protein expression level of RIPK4 and β -catenin in SKOV3 and HO8910 cells after transfection.

Article Snippet: The membranes were blocked with Tris-buffered saline with 0.1% ( v / v ) Tween-20 (TBST) containing 5% ( w / v ) nonfat milk for an hour at room temperature and incubated with primary antibodies for RIPK4 (A8495; 1 : 1000; ABclonal Technology, Wuhan, China), β -catenin (ab16051; 1 : 1000; Abcam, Cambridge, MA, USA), or β -actin (1 : 1000; Proteintech, Chicago, IL, USA) overnight at 4°C.

Techniques: Expressing, Transfection

Journal: Scientific Reports

Article Title: Increased RIPK4 expression is associated with progression and poor prognosis in cervical squamous cell carcinoma patients

doi: 10.1038/srep11955

Figure Lengend Snippet: ( a ) qRT-PCR analysis of RIPK4 mRNA expression in 101 fresh CSCC tissues (T) and 30 paracancerous tissues (N). Expression levels were normalized to those of EFF1A1. ( b ) RIPK4 protein levels were determined by western blot in 8-paired CSCC tissues (T) and paracancerous tissues (N). GAPDH was used as a loading control. ( c ) Quantitative analysis of RIPK4 protein levels by western blot was presented as median ± interquartile. ( d ) RIPK4 immunohistochemical staining in representative cases from each cervical lesion. The scale bar represents 50 μm. ( e ) Expression of RIPK4 in each cervical lesion based on immunohistochemical scoring, as determined by the percentage of positive cells with strong staining. Values are the median with interquartile range.* P < 0.05 by the Wilcoxon rank-sum test.

Article Snippet: A rabbit antibody against RIPK4 (1:500 dilution; Abgent, San Diego, USA) was used to detect RIPK4 protein, and a rabbit antibody against GAPDH protein (1:3000 dilution; Sigma, St. Louis, Mo., USA) was used as an internal control.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Immunohistochemical staining, Staining

Journal: Scientific Reports

Article Title: Increased RIPK4 expression is associated with progression and poor prognosis in cervical squamous cell carcinoma patients

doi: 10.1038/srep11955

Figure Lengend Snippet: RIPK4 expression and clinicopathological variables in CSCC.

Article Snippet: A rabbit antibody against RIPK4 (1:500 dilution; Abgent, San Diego, USA) was used to detect RIPK4 protein, and a rabbit antibody against GAPDH protein (1:3000 dilution; Sigma, St. Louis, Mo., USA) was used as an internal control.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Increased RIPK4 expression is associated with progression and poor prognosis in cervical squamous cell carcinoma patients

doi: 10.1038/srep11955

Figure Lengend Snippet: Summary of immunohistochemistry data for cervical samples and clinical value of RIPK4, p16 INK4a and Ki-67 in relationship to diagnoses of HSIL.

Article Snippet: A rabbit antibody against RIPK4 (1:500 dilution; Abgent, San Diego, USA) was used to detect RIPK4 protein, and a rabbit antibody against GAPDH protein (1:3000 dilution; Sigma, St. Louis, Mo., USA) was used as an internal control.

Techniques: Immunohistochemistry

Journal: Scientific Reports

Article Title: Increased RIPK4 expression is associated with progression and poor prognosis in cervical squamous cell carcinoma patients

doi: 10.1038/srep11955

Figure Lengend Snippet: ( a ) Immunohistochemical staining of RIPK4 in representative squamous cell carcinoma cases with low and high RIPK4 expression, respectively (magnification, 400×). ( b ) OS curves: 198 patients with low and high expression levels of RIPK4; ( c ) DFS curves: 198 patients with low and high expression levels of RIPK4. Disease-free survival analysis of RIPK4 expression in patients with ( d ) stage IB1, ( e ) LN metastasis or ( f ) histological grade III.

Article Snippet: A rabbit antibody against RIPK4 (1:500 dilution; Abgent, San Diego, USA) was used to detect RIPK4 protein, and a rabbit antibody against GAPDH protein (1:3000 dilution; Sigma, St. Louis, Mo., USA) was used as an internal control.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Scientific Reports

Article Title: Increased RIPK4 expression is associated with progression and poor prognosis in cervical squamous cell carcinoma patients

doi: 10.1038/srep11955

Figure Lengend Snippet: Multivariate Cox regression analysis for survival in CSCC.

Article Snippet: A rabbit antibody against RIPK4 (1:500 dilution; Abgent, San Diego, USA) was used to detect RIPK4 protein, and a rabbit antibody against GAPDH protein (1:3000 dilution; Sigma, St. Louis, Mo., USA) was used as an internal control.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Increased RIPK4 expression is associated with progression and poor prognosis in cervical squamous cell carcinoma patients

doi: 10.1038/srep11955

Figure Lengend Snippet: ( a, b ) Real-time quantitative PCR analysis of RIPK4 mRNA expression and western blot analysis of RIPK4 protein expression in SiHa and Caski cells transfected with specific siRNAs targeting RIPK4 for 48 h. ( c, d ) Colony formation capacity of SiHa and Caski cells transfected with RIPK4 siRNAs and control siRNA were analyzed by colony formation assay. ( e ) Inhibition of SiHa cell proliferation by RIPK4 siRNAs tested by MTT assays. ( f ) Inhibition of Caski cell proliferation by RIPK4 siRNAs as measured by MTT assays. Values are the mean ± s.d. of three independent experiments. * P < 0.05 relative to the control.

Article Snippet: A rabbit antibody against RIPK4 (1:500 dilution; Abgent, San Diego, USA) was used to detect RIPK4 protein, and a rabbit antibody against GAPDH protein (1:3000 dilution; Sigma, St. Louis, Mo., USA) was used as an internal control.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Transfection, Colony Assay, Inhibition

Journal: Scientific Reports

Article Title: Increased RIPK4 expression is associated with progression and poor prognosis in cervical squamous cell carcinoma patients

doi: 10.1038/srep11955

Figure Lengend Snippet: ( a ) Inhibition of SiHa and Caski cell migration by RIPK4 siRNAs as determined by migration assays. ( b ) Inhibition of SiHa and Caski cell invasion by RIPK4 siRNAs, as determined by Matrigel invasion assays. ( c, d ) RIPK4 was downregulated in SiHa and Caski cells, and the mRNA expression levels of Vimentin, MMP2 and Fibronectin were detected by qRT-PCR. GAPDH was used as an endogenous control. Values are the mean ± s.d. of three independent experiments. * P < 0.05 relative to the control.

Article Snippet: A rabbit antibody against RIPK4 (1:500 dilution; Abgent, San Diego, USA) was used to detect RIPK4 protein, and a rabbit antibody against GAPDH protein (1:3000 dilution; Sigma, St. Louis, Mo., USA) was used as an internal control.

Techniques: Inhibition, Migration, Expressing, Quantitative RT-PCR

Journal: The EMBO Journal

Article Title: Phosphorylation of Pkp1 by RIPK 4 regulates epidermal differentiation and skin tumorigenesis

doi: 10.15252/embj.201695679

Figure Lengend Snippet: H/E staining of skin tumors generated from WT and RIPK4 cKO animals upon DMBA/TPA treatment. Scale bar = 200 μm.

Article Snippet: Rabbit polycloncal antibody against RIPK4 was purchased from Abnova (Taiwan).

Techniques: Staining, Generated